《植物生理学报》 2015, 51(11): 1873-1879

2-氧化戊二酸依赖的双加氧酶基因F6’H1促进拟南芥叶片衰老

胡有贞^1,2,*, 王雅欣²

¹华东师范大学生命科学学院, 上海200241; ²美国康奈尔大学园艺系, 美国纽约州伊萨卡14853

通信作者：胡有贞；E-mail: hyz1106@126.com；Tel: 021-54341012

摘　要：

拟南芥2-氧化戊二酸依赖的双加氧酶基因F6’H1缺失突变体F6’H1出现叶片衰老延迟表型。利用根癌农杆菌介导的花序浸染法转化拟南芥, 再以抗性筛选方法获得了F6’H1基因诱导过量表达拟南芥植株, 地塞米松(DEX)喷施处理24 h能显著诱导F6’H1基因上调表达, F6’H1基因的过量表达使转基因拟南芥叶片衰老提前。结果表明, 拟南芥2-氧化戊二酸依赖的双加氧酶基因F6’H1正向调控叶片衰老。

关键词：拟南芥; 2-氧化戊二酸依赖的双加氧酶基因F6’H1; 叶片衰老

收稿：2015-06-16   修定：2015-09-15

资助：国家留学基金委员会(留金发[2012]3013)。
致谢 康乃尔大学园艺系Susheng Gan教授为本研究提供指导和研究条件, 华东师范大学生命科学学院许玲教授在文章撰写方面给予宝贵建议。

2-Oxoglutarate Dependent Dioxygenase Gene F6’H1 of Arabidopsis thaliana Promote Leaf Senescence

HU You-Zhen^1,2,*, WANG Ya-Xin²

¹School of Life Sciences, East China Normal University, Shanghai 200241, China; ²Department of Horticulture, University Cornell, Ithaca, New York 14853, USA

Corresponding author: HU You-Zhen; E-mail: hyz1106@126.com; Tel: 021-54341012

Abstract:

2-oxoglutarate dependent dioxygenase gene F6’H1 knockout mutant F6’H1 exhibited significantly delayed leaf senescence in Arabidopsis thaliana. A dexamethasone (DEX, a synthetic glucocorticoid) inducible overexpression system containing the F6’H1 gene was transformed into wild-type plants through floral dipping method, which was mediated by Agrobacterium tumefaciens. The transgenic plants resistant to hygromycin and kanamycin were obtained. The transcription of F6’H1 in transgenic leaves was increased after DEX spraying whole plant. Comparing with the wild-type plants, the precocious leaf yellowing were observed 5 d after the DEX treatment in F6’H1 inducible overexpression lines. Taken together, 2-oxoglutarate dependent dioxygenase gene F6’H1 positively regulate leaf senescence of Arabidopsis thaliana.

Key words: Arabidopsis thaliana; 2-oxoglutarate dependent dioxygenase gene F6’H1; leaf senescence